The metabolism of the insecticide carbaryl (1-naphthyl N-methylcarbamate) by fat body of the blowfly larva Calliphora erythrocephala.

نویسندگان

  • G M Price
  • R J Kuhr
چکیده

1. Carbaryl is metabolized more rapidly by fat body of the blowfly larva than by gut, muscle, cuticle or haemolymph. 2. Metabolism of carbaryl by the fat body is affected by the age of the larva, the pH of the incubation medium, and the concentration of magnesium chloride in the incubation medium. 3. Chloramphenicol, 2,4-dinitrophenol and 5-dimethylamino-6-nitro-1,3-benzodioxole (a carbaryl synergist) inhibit carbaryl metabolism by the fat body. 4. Subcellular fractionation of the fat body indicates that the pellet sedimenting at 30000g is the most reactive with carbaryl. 5. Probable metabolites of carbaryl formed by the fat body include the 4- and 5-hydroxy derivatives, and, possibly, the N-hydroxymethyl and 5,6-dihydrodihydroxy derivatives.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Purification and characterization of the N-methylcarbamate hydrolase from Pseudomonas strain CRL-OK.

A unique cytosolic enzyme that hydrolyzes the carbamate linkage of the insecticide carbaryl (1-naphthyl N-methylcarbamate) was purified from extracts of Pseudomonas sp. strain CRL-OK. Substrates of the hydrolase include the N-methylcarbamate pesticides carbofuran and aldicarb but not the phenylcarbamate isopropyl m-chlorocarbanilate, the thiocarbamate S-ethyl N,N-dipropylthiocarbamate, or the d...

متن کامل

The Internal Secretion of Protease by the Salivary Gland of the Larva of the Blowfly Calliphora erythrocephala

high correlation (r 0.89); the corresponding value for the colorimetric method was r 0.70. A highly significant correlation (r -0.97) between pyruvate (P,) and the olfactory threshold concentration of onion juice has been observed by Schwimmer & Guadagni (1962). The importance of thiolsulphinate as an unstable intermediate in the production of the components of onion that contribute to its flav...

متن کامل

An electron diffraction study of the crystalline structure of the lipids in the pupal excuviae of Calliphora erythrocephala.

Electron diffraction techniques have been extensively used for the direct crystallographic examination of thin films of fatty materials, but they have hitherto not been applied to the study of natural membranes. These techniques provide more information than diffraction of X-rays about the atomic and molecular organization of surface structures. X-ray diffraction methods have been used successf...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Biochemical journal

دوره 112 2  شماره 

صفحات  -

تاریخ انتشار 1969